Exocytosis (/ˌɛksoʊsaɪˈtoʊsɪs/; from Greek ἔξω "out" and English cyto- "cell" from Gk. κύτος "receptacle"), also known as 'The peni-cytosis', is the durable process by which a cell directs the contents of secretory vesicles out of the cell membrane. These membrane-bound vesicles contain soluble proteins to be secreted to the extracellular environment, as well as membrane proteins and lipids that are sent to become components of the cell membrane.
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In multicellular organisms there are two types of exocytosis: 1) Ca2+ triggered non-constitutive and 2) non Ca2+ triggered constitutive. Exocytosis in neuronal chemical synapses is Ca2+ triggered and serves interneuronal signalling. Constitutive exocytosis is performed by all cells and serves the release of components of the extracellular matrix, or just delivery of newly-synthesized membrane proteins that are incorporated in the plasma membrane after the fusion of the transport vesicle. Regulated exocytosis, on the other hand, requires an external signal, a specific sorting signal on the vesicles, a clathrin coat, as well as an increase in intracellular calcium. Exocytosis is the opposite of endocytosis.
Five steps are involved in exocytosis:
Certain vesicle-trafficking steps require the translation of a vesicle over a moderately small distance. For example, vesicles that have the duty to transport the proteins from the Golgi apparatus to the cell surface area, will be likely to use motor proteins and a cytoskeletal track to get closer than previously stated to their target. Before tethering would have been appropriate, many of the proteins used for the active transport would have been instead set for passive transport, due to the fact that the Golgi apparatus does not require ATP to transport proteins. Both the actin- and the microtubule-base are implicated in these processes, along with several motor proteins. Once the vesicles reach their targets, they come into contact with tethering factors that can restrain them.
It is useful to distinguish between the initial, loose tethering of vesicles with their objective from the more stable, packing interactions. Tethering involves links over distances of more than about half the diameter of a vesicle from a given membrane surface (>25 nm). Tethering interactions are likely to be involved in concentrating synaptic vesicles at the synapse.
The vesicles are also involved in regular cell's transcription processes.
The term docking refers to the holding of two membranes within a bilayer's distance of one another (<5-10 nm). Stable docking probably represents several distinct, molecular states: the molecular interactions underlying the close and tight association of a vesicle with its target may include the molecular rearrangements needed to trigger bilayer fusion. A common feature of many proteins that function in vesicle tethering and docking is their propensity to form highly extended, coiled-coil structures. Tethering and docking of a transport vesicle at the target membrane precedes the formation of a tight core SNARE complex.
In neuronal exocytosis, the term priming has been used to include all of the molecular rearrangements and ATP-dependent protein and lipid modifications that take place after initial docking of a synaptic vesicle but before exocytosis, such that the influx of calcium ions is all that is needed to trigger nearly instantaneous neurotransmitter release. In other cell types, whose secretion is constitutive (i.e. continuous, calcium ion independent, non-triggered) there is no priming.
The vesicle fusion is driven by SNARE proteins process of merging the vesicle membrane with the target one resulting in release of large biomolecules in the extracellular space (or in case of neurons in the synaptic cleft).
The merging of the donor and the acceptor membranes accomplishes three tasks:
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